Real-time analysis of skin biopsy specimens and Mohs surgical margin using 2-photon fluorescence microscopy with piston-based specimen holder

Chi Z. R. Huang; Vincent D. Ching-Roa; Connor M Heckman; Sherrif F. Ibrahim; Bruce R. Smoller; Michael G Giacomelli

doi:10.1117/12.3001266

13 March 2024 Real-time analysis of skin biopsy specimens and Mohs surgical margin using 2-photon fluorescence microscopy with piston-based specimen holder

Chi Z. R. Huang, Vincent D. Ching-Roa, Connor M Heckman, Sherrif F. Ibrahim, Bruce R. Smoller, Michael G Giacomelli

Author Affiliations +

Proceedings Volume PC12816, Photonics in Dermatology and Plastic Surgery 2024; PC128160O (2024) https://doi.org/10.1117/12.3001266
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In this study, we developed an improved piston-based specimen holder to provide even pressure distribution across an irregular tissue surface. A series of support fixtures are also developed to facilitate the pressure distribution from the piston to image specimens with small contact area relative to thickness such as bisected shave skin biopsies. Using this capability, we demonstrate imaging of tall and narrow biopsy specimens with precise coregistration to conventional histology as well as rapid imaging of Mohs margins during surgery.

Conference Presentation

Citation Download Citation

Chi Z. R. Huang, Vincent D. Ching-Roa, Connor M Heckman, Sherrif F. Ibrahim, Bruce R. Smoller, and Michael G Giacomelli "Real-time analysis of skin biopsy specimens and Mohs surgical margin using 2-photon fluorescence microscopy with piston-based specimen holder", Proc. SPIE PC12816, Photonics in Dermatology and Plastic Surgery 2024, PC128160O (13 March 2024); https://doi.org/10.1117/12.3001266

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