Calibration of delayed fluorescence of protoporphyrin IX for in vivo quantification of oxygen

Arthur F. Pétusseau; Petr Bruza; Brian Pogue

doi:10.1117/12.3004506

13 March 2024 Calibration of delayed fluorescence of protoporphyrin IX for in vivo quantification of oxygen

Arthur F. Pétusseau, Petr Bruza, Brian Pogue

Proceedings Volume PC12823, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXII; PC1282309 (2024) https://doi.org/10.1117/12.3004506
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Oxygen's pivotal role in biology extends to oncology, affecting photodynamic therapy, radiotherapy, and chemotherapy efficacy. While invasive oxygen electrodes are the standard for in vivo measurement, they lack cellular information and are limited to single point measurements. Alternatively, noninvasive optical oxygen probes, quenching in the presence of oxygen, offer fast spatially resolved insights. However, limited FDA approval restricts their clinical use. In this study, we explore the use of the endogenous molecule protoporphyrin IX (PpIX), to measure tissue oxygen, using a novel fluorescence imaging approach. Our goal is to calibrate the PpIX ratiometric signal for precise pO2 measurements. We demonstrate the correlation between PpIX ratiometric signal and tissue oxygen and present an estimated correlation curve. This advancement in non-invasive intracellular measurement holds profound implications across medical disciplines, and particularly for PDT.

Conference Presentation

Citation Download Citation

Arthur F. Pétusseau, Petr Bruza, and Brian Pogue "Calibration of delayed fluorescence of protoporphyrin IX for in vivo quantification of oxygen", Proc. SPIE PC12823, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXII, PC1282309 (13 March 2024); https://doi.org/10.1117/12.3004506

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KEYWORDS

Oxygen

Calibration

In vivo imaging

Fluorescence

Electrodes

Molecules

Photodynamic therapy

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