3D dynamic contrast optical coherence tomography for ciliary beating monitoring in airway organoids

Chao Ren; Daniel Yang; Senyue Hao; Fei Wang; Kibeom Park; Leyao Wang; Chao Zhou

doi:10.1117/12.3005684

13 March 2024 3D dynamic contrast optical coherence tomography for ciliary beating monitoring in airway organoids

Chao Ren, Daniel Yang, Senyue Hao, Fei Wang, Kibeom Park, Leyao Wang, Chao Zhou

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC128302L (2024) https://doi.org/10.1117/12.3005684
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Airway organoid is a three-dimensional (3D) epithelial models for human airway where ciliary behaviors are important to indicate their health state. A real-time noninvasive imaging technique to assess the ciliary movement in airway organoids in vitro is highly demanded. Here we propose a new imaging approach to monitor the ciliary beating of airway organoids noninvasively using dynamic contrast optical coherence tomography (DyC-OCT). We employed DyC-OCT to measure the ciliary beating frequency (CBF) and to highlight ciliary beating clusters distribution in 3D. With DyC-OCT, we monitored the morphology change in ciliary beating clusters as well as the change in CBF from the same organoids during the respiratory syncytial virus (RSV) infection. The capability of DyC-OCT in ciliary beating monitoring shows its potential for respiratory disease diagnosis and pathogen assay.

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Citation Download Citation

Chao Ren, Daniel Yang, Senyue Hao, Fei Wang, Kibeom Park, Leyao Wang, and Chao Zhou "3D dynamic contrast optical coherence tomography for ciliary beating monitoring in airway organoids", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC128302L (13 March 2024); https://doi.org/10.1117/12.3005684

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KEYWORDS

Optical coherence tomography

Optical sensing

3D modeling

Pathogens

Pulmonary disorders

3D image processing

Diagnostics

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