Dynamics of morphological and metabolic development of human 3D printed skin equivalent monitored by dual-mode OCT and fluorescence spectrometry system

Arooj Khalid; Viktor Dremin; Ayman El-Tamer; Maria Surnina; Celine Lancelot; Edik Rafailov; Sergei Sokolovski

doi:10.1117/12.3013009

13 March 2024 Dynamics of morphological and metabolic development of human 3D printed skin equivalent monitored by dual-mode OCT and fluorescence spectrometry system

Arooj Khalid, Viktor Dremin, Ayman El-Tamer, Maria Surnina, Celine Lancelot, Edik Rafailov, Sergei Sokolovski

Author Affiliations +

Proceedings Volume PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI; PC128410L (2024) https://doi.org/10.1117/12.3013009
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The 3D architecture of native human skin is a crucial requirement in investigating the skin in health and pathologies, wound healing, and assessment of skin cosmetic and care product safety and personalized skin disease treatments. In this research, we used human skin cell lines to build fullthickness skin equivalents (FSE). Engineering artificial 3D tissue models is a known challenge in molecular biology and regenerative medicine not only due to difficulties in fabricating high-resolution scaffolds but in nondamaging monitoring of artificial tissue growth in dynamics. Here we architected 3D laser-printed scaffolds for the comfortable growth and maturation of the FSEs and also developed and validated custom-built combined fluorescence spectrometry (FS) and optical coherence tomography (OCT) imaging system for indestructible assessing metabolism and morphology of developing 3D human FSEs. This system demonstrated high sensitivity in detecting fluorescence from nicotinamide adenine dinucleotide (NADH) and riboflavin 5’-adenosine diphosphate (FAD) in solutions and cell suspensions, and high-resolution imaging of FSE morphology. Thus, our developed FSE on 3D laser-printed scaffolds and dual-mode optical system can be used in the future for nondamaging assessment of metabolism, maturation, and viability of 3D tissue models in growth dynamics.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Arooj Khalid, Viktor Dremin, Ayman El-Tamer, Maria Surnina, Celine Lancelot, Edik Rafailov, and Sergei Sokolovski "Dynamics of morphological and metabolic development of human 3D printed skin equivalent monitored by dual-mode OCT and fluorescence spectrometry system", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC128410L (13 March 2024); https://doi.org/10.1117/12.3013009

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KEYWORDS

Skin

3D modeling

3D image processing

3D printing

Optical coherence tomography

Imaging systems

Photonics systems

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