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13 March 2024 Super-resolution and lifetime imaging of nano-emitters
Duncan P. Ryan, James H. Werner, Rebecca Sandoval, Bibek Dhakal, Alan K. Van Orden, Martin P. Gelfand
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Proceedings Volume PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; PC128490Q (2024) https://doi.org/10.1117/12.3000581
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Using a novel imaging device—NCam—fluorophore lifetime measurements can be captured simultaneously with wide-field microscopy methods. Because NCam records single-photon events with spatial and temporal information, the localization precision can be improved compared to camera-based imaging or scanning confocal instruments. We demonstrate this new imaging capability by examining the fluorescence behavior of quantum dots.
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Duncan P. Ryan, James H. Werner, Rebecca Sandoval, Bibek Dhakal, Alan K. Van Orden, and Martin P. Gelfand "Super-resolution and lifetime imaging of nano-emitters", Proc. SPIE PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, PC128490Q (13 March 2024); https://doi.org/10.1117/12.3000581
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KEYWORDS
Super resolution
Fluorescence lifetime imaging
Cameras
Fluorophores
Microscopy
Quantum emitters
Quenching
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