Comparing the accuracies of quantitative phase microscopies (QPM)

Guillaume Baffou; Anne Sentenac; Patrick Chaumet; Guillaume Maire

doi:10.1117/12.3001277

13 March 2024 Comparing the accuracies of quantitative phase microscopies (QPM)

Guillaume Baffou, Anne Sentenac, Patrick Chaumet, Guillaume Maire

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC1285207 (2024) https://doi.org/10.1117/12.3001277
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

In this contribution, we aim to compare the accuracy of a large variety of quantitative phase imaging (QPI) techniques (phase shifting, off axis, etc) in terms of trueness and precision. For this purpose, we developed a numerical toolbox capable of computing the electromagnetic field at the sample plane of arbitrarily complex microscopes, in the presence of any microscale object at the sample plane. The discussions are based on the numerical imaging of nanoparticles, micro-beads, bacteria, 2D materials and mammalian cells. This way, we could discuss the benefits and limitations of all the existing QPI techniques to help better chose the right tool as a function of the application.

Conference Presentation

Citation Download Citation

Guillaume Baffou, Anne Sentenac, Patrick Chaumet, and Guillaume Maire "Comparing the accuracies of quantitative phase microscopies (QPM)", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC1285207 (13 March 2024); https://doi.org/10.1117/12.3001277

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