Mirror slides for high-sensitivity cell and tissue fluorescence imaging

Eric Le Moal; Emmanuel Fort; Sandrine Lévêque-Fort; Anne Janin; Hideyuki Murata; Fabrice P. Cordelieres; Marie-Pierre P. Fontaine-Aupart; Christian Ricolleau

doi:10.1117/1.2722730

1 March 2007 Mirror slides for high-sensitivity cell and tissue fluorescence imaging

Eric Le Moal, Emmanuel Fort, Sandrine Lévêque-Fort, Anne Janin, Hideyuki Murata, Fabrice P. Cordelieres, Marie-Pierre P. Fontaine-Aupart, Christian Ricolleau

Author Affiliations +

Journal of Biomedical Optics, Vol. 12, Issue 2, 024030 (March 2007). https://doi.org/10.1117/1.2722730

Abstract

Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Eric Le Moal, Emmanuel Fort, Sandrine Lévêque-Fort, Anne Janin, Hideyuki Murata, Fabrice P. Cordelieres, Marie-Pierre P. Fontaine-Aupart, and Christian Ricolleau "Mirror slides for high-sensitivity cell and tissue fluorescence imaging," Journal of Biomedical Optics 12(2), 024030 (1 March 2007). https://doi.org/10.1117/1.2722730

Published: 1 March 2007

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY