In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B

Pascale Vérant; Clément Ricard; Raphael Serduc; Jean-Claude A. Vial; Boudewijn P. J. van der Sanden

doi:10.1117/1.3041163

1 November 2008 In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B

Pascale Vérant, Clément Ricard, Raphael Serduc, Jean-Claude A. Vial, Boudewijn P. J. van der Sanden

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Journal of Biomedical Optics, Vol. 13, Issue 6, 064028 (November 2008). https://doi.org/10.1117/1.3041163

Abstract

Staining and imaging glial cells in vivo while observing the microvasculature could help understand brain physiology, namely neuronal-glial-vascular communication. Two-photon excitation microscopy provides a means to monitor these interactions at the cellular level in living animals, but the cells of interest must be fluorescent. Injecting dyes intravenously is a rapid and quasi noninvasive method to stain cells in the brain. It necessitates that the dye is soluble in the blood plasma and crosses the blood brain barrier (BBB). We demonstrate here, using two-photon imaging, that sulforhodamine B (SRB) crosses the BBB and stains in vivo, specifically mouse astrocytes. This is confirmed by experiments on primary neurons and astrocytes cultures showing the preferential SRB staining of the latter. SRB is rapidly eliminated from the blood, which allows repeated injections in longitudinal studies.

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Pascale Vérant, Clément Ricard, Raphael Serduc, Jean-Claude A. Vial, and Boudewijn P. J. van der Sanden "In vivo staining of neocortical astrocytes via the cerebral microcirculation using sulforhodamine B," Journal of Biomedical Optics 13(6), 064028 (1 November 2008). https://doi.org/10.1117/1.3041163

Published: 1 November 2008

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