In vitro and in vivo three-dimensional velocity vector measurement by three-beam spectral-domain Doppler optical coherence tomography

Wolfgang Trasischker; René M. Werkmeister; Stefan Zotter; Bernhard Baumann; Teresa Torzicky; Michael Pircher; Christoph K. Hitzenberger

doi:10.1117/1.JBO.18.11.116010

18 November 2013 In vitro and in vivo three-dimensional velocity vector measurement by three-beam spectral-domain Doppler optical coherence tomography

Wolfgang Trasischker, René M. Werkmeister, Stefan Zotter, Bernhard Baumann, Teresa Torzicky, Michael Pircher, Christoph K. Hitzenberger

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Funded by: European Union, Austrian Science Fund

Journal of Biomedical Optics, Vol. 18, Issue 11, 116010 (November 2013). https://doi.org/10.1117/1.JBO.18.11.116010

Abstract

We developed a three-beam Doppler optical coherence tomography (OCT) system that enables measurement of the velocity vector of moving particles in three-dimensions (3-D). The spatial orientation as well as the magnitude of motion can be determined without prior knowledge of the geometry of motion. The system combines three spectral-domain OCT interferometers whose sample beams are focused at the sample by a common focusing lens at three different angles. This provides three spatially independent velocity components simultaneously from which the velocity vector can be reconstructed. We demonstrate the system in a simple test object (rotating disc), a flow phantom, and for blood flow measurements in the retina of a healthy human subject. Measurements of blood flow at a venous bifurcation achieve a good agreement between in- and outflow and demonstrate the reliability of the method.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Wolfgang Trasischker, René M. Werkmeister, Stefan Zotter, Bernhard Baumann, Teresa Torzicky, Michael Pircher, and Christoph K. Hitzenberger "In vitro and in vivo three-dimensional velocity vector measurement by three-beam spectral-domain Doppler optical coherence tomography," Journal of Biomedical Optics 18(11), 116010 (18 November 2013). https://doi.org/10.1117/1.JBO.18.11.116010

Published: 18 November 2013

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