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24 June 2013 Direct high-resolution label-free imaging of cellular nanostructure dynamics in living cells
Chaejeong Heo, Sohee Lee, Si Young Lee, Mun Seok Jeong, Young Hee Lee, Minah Suh
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Journal of Biomedical Optics, Vol. 18, Issue 6, 066016 (June 2013). https://doi.org/10.1117/1.JBO.18.6.066016
Abstract
We report the application of an optical microscope equipped with a high-resolution dark-field condenser for detecting dynamic responses of cellular nanostructures in real time. Our system provides an easy-to-use technique to visualize biological specimens without any staining. This system can visualize the dynamic behavior of nanospheres and nanofibers, such as F-actin, at the leading edges of adjacent neuronal cells. We confirmed that the nanofibers imaged with this high-resolution optical microscopic technique are F-actin by using fluorescence microscopy after immunostaining the F-actin of fixed cells. Furthermore, cellular dynamics are enhanced by applying noncontact electric field stimulation through a transparent graphene electric field stimulator. High-resolution label-free optical microscopy enables the visualization of nanofiber dynamics initiated by filopodial nanofiber contacts. In conclusion, our optical microscopy system allows the visualization of nanoscale cellular dynamics under various external stimuli in real time without specific staining.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Chaejeong Heo, Sohee Lee, Si Young Lee, Mun Seok Jeong, Young Hee Lee, and Minah Suh "Direct high-resolution label-free imaging of cellular nanostructure dynamics in living cells," Journal of Biomedical Optics 18(6), 066016 (24 June 2013). https://doi.org/10.1117/1.JBO.18.6.066016
Published: 24 June 2013
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KEYWORDS
Imaging systems
Nanostructures
Nanofibers
Visualization
Microscopy
Fermium
Frequency modulation
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