CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 18 > Issue 8 > Article
Open Access
2 August 2013 Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels
Yang Pu, Jianpeng Xue, Wubao Wang, Baogang Xu, Yueqing Gu, Rui Tang, Ellen Ackerstaff, Jason A. Koutcher M.D., Samuel Achilefu, Robert R. Alfano
Author Affiliations +
Journal of Biomedical Optics, Vol. 18, Issue 8, 087002 (August 2013). https://doi.org/10.1117/1.JBO.18.8.087002
Abstract
The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2013/$25.00 © 2013 SPIE
Citation Download Citation
Yang Pu, Jianpeng Xue, Wubao Wang, Baogang Xu, Yueqing Gu, Rui Tang, Ellen Ackerstaff, Jason A. Koutcher M.D., Samuel Achilefu, and Robert R. Alfano "Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels," Journal of Biomedical Optics 18(8), 087002 (2 August 2013). https://doi.org/10.1117/1.JBO.18.8.087002
Published: 2 August 2013
JOURNAL ARTICLE
 6 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 37 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Cancer
Luminescence
Prostate cancer
Principal component analysis
Fluorescence spectroscopy
Solids
Spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top