Evaluation of fractional photothermolysis effect in a mouse model using nonlinear optical microscopy

Han-Wen Guo; Te-Yu Tseng; Chen-Yuan Dong; Tsung-Hua Tsai

doi:10.1117/1.JBO.19.7.075004

14 July 2014 Evaluation of fractional photothermolysis effect in a mouse model using nonlinear optical microscopy

Han-Wen Guo, Te-Yu Tseng, Chen-Yuan Dong, Tsung-Hua Tsai

Author Affiliations +

Journal of Biomedical Optics, Vol. 19, Issue 7, 075004 (July 2014). https://doi.org/10.1117/1.JBO.19.7.075004

Abstract

Fractional photothermolysis (FP) induces discrete columns of photothermal damage in skin dermis, thereby promoting collagen regeneration. This technique has been widely used for treating wrinkles, sun damage, and scar. In this study, we evaluate the potential of multiphoton microscopy as a noninvasive imaging modality for the monitoring of skin rejuvenation following FP treatment. The dorsal skin of a nude mouse underwent FP treatment in order to induce microthermal zones (MTZs). We evaluated the effect of FP on skin remodeling at 7 and 14 days after treatment. Corresponding histology was performed for comparison. After 14 days of FP treatment at 10 mJ, the second harmonic generation signal recovered faster than the skin treated with 30 mJ, indicating a more rapid regeneration of dermal collagen at 10 mJ. Our results indicate that nonlinear optical microscopy is effective in detecting the damaged areas of MTZ and monitoring collagen regeneration following FP treatment.

Citation Download Citation

Han-Wen Guo, Te-Yu Tseng, Chen-Yuan Dong, and Tsung-Hua Tsai "Evaluation of fractional photothermolysis effect in a mouse model using nonlinear optical microscopy," Journal of Biomedical Optics 19(7), 075004 (14 July 2014). https://doi.org/10.1117/1.JBO.19.7.075004

Published: 14 July 2014

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY