CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 19 > Issue 8 > Article
Open Access
27 August 2014 Re-evaluation of biotin-streptavidin conjugation in Förster resonance energy transfer applications
Bahar Saremi, Ming-Yuan Wei, Yuan Liu, Bingbing Cheng, Baohong Yuan
Author Affiliations +
Journal of Biomedical Optics, Vol. 19, Issue 8, 085008 (August 2014). https://doi.org/10.1117/1.JBO.19.8.085008
Abstract
Bioaffinity conjugation between streptavidin (SA) and biotin has been widely used to link donors and acceptors for investigating the distance-dependent Förster resonance energy transfer (FRET). When studying a commonly used FRET system of (QD-SA)-(biotin-DNA-dye) [donor: quantum dot (QD); acceptor: small organic fluorescent dye; and linker: deoxyribose nucleic acid (DNA) molecule via SA-biotin conjugation], however, a contradictory finding was recently reported in the literature. It was found that the FRET lost its dependence on the number of DNA base pairs when using a phosphate-buffered saline (PBS) solution. We found that the conflicted results were caused by the ionic strength of the adopted buffer solutions. Our results suggest that the dependent FRET on the number of DNA bases is favorable in a low-ionic-strength buffer, whereas in relatively high-ionic-strength buffers, the FRET loses the DNA length dependence. We propose that the independence is mainly caused by the conformational change of DNA molecules from a stretched to a coiled mode when the cations in the high-ionic-strength buffer neutralize the negatively charged backbone of DNA molecules, thereby bringing the acceptors close to the donors.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2014/$25.00 © 2014 SPIE
Citation Download Citation
Bahar Saremi, Ming-Yuan Wei, Yuan Liu, Bingbing Cheng, and Baohong Yuan "Re-evaluation of biotin-streptavidin conjugation in Förster resonance energy transfer applications," Journal of Biomedical Optics 19(8), 085008 (27 August 2014). https://doi.org/10.1117/1.JBO.19.8.085008
Published: 27 August 2014
JOURNAL ARTICLE
 12 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 5 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Fluorescence resonance energy transfer
Luminescence
Molecules
Computed tomography
Resonance energy transfer
Optical filters
Bandpass filters
CHORUS Article. This article was made freely available starting 27 August 2015
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top