Photoacoustic imaging of human lymph nodes with endogenous lipid and hemoglobin contrast

James A. Guggenheim; Thomas J. Allen; Andrew Plumb; Edward Z. Zhang; Manuel Rodriguez-Justo; Shonit Punwani; Paul C. Beard

doi:10.1117/1.JBO.20.5.050504

26 May 2015 Photoacoustic imaging of human lymph nodes with endogenous lipid and hemoglobin contrast

James A. Guggenheim, Thomas J. Allen, Andrew Plumb, Edward Z. Zhang, Manuel Rodriguez-Justo, Shonit Punwani, Paul C. Beard

Author Affiliations +

Journal of Biomedical Optics, Vol. 20, Issue 5, 050504 (May 2015). https://doi.org/10.1117/1.JBO.20.5.050504

Abstract

Lymph nodes play a central role in metastatic cancer spread and are a key clinical assessment target. Abnormal node vascularization, morphology, and size may be indicative of disease but can be difficult to visualize with sufficient accuracy using existing clinical imaging modalities. To explore the potential utility of photoacoustic imaging for the assessment of lymph nodes, images of ex vivo samples were obtained at multiple wavelengths using a high-resolution three-dimensional photoacoustic scanner. These images showed that hemoglobin based contrast reveals nodal vasculature and lipid-based contrast reveals the exterior node size, shape, and boundary integrity. These two sources of complementary contrast may allow indirect observation of cancer, suggesting a future role for photoacoustic imaging as a tool for the clinical assessment of lymph nodes.

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James A. Guggenheim, Thomas J. Allen, Andrew Plumb, Edward Z. Zhang, Manuel Rodriguez-Justo, Shonit Punwani, and Paul C. Beard "Photoacoustic imaging of human lymph nodes with endogenous lipid and hemoglobin contrast," Journal of Biomedical Optics 20(5), 050504 (26 May 2015). https://doi.org/10.1117/1.JBO.20.5.050504

Published: 26 May 2015

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KEYWORDS

Lymphatic system

Photoacoustic imaging

Cancer

Absorption

Photoacoustic spectroscopy

Sensors

Visualization

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