Evaluating anesthetic protocols for functional blood flow imaging in the rat eye

Eric M. Moult; WooJhon Choi; David A. Boas; Bernhard Baumann; Allen C. Clermont; Edward P. Feener; James G. Fujimoto

doi:10.1117/1.JBO.22.1.016005

5 January 2017 Evaluating anesthetic protocols for functional blood flow imaging in the rat eye

Eric M. Moult, WooJhon Choi, David A. Boas, Bernhard Baumann, Allen C. Clermont, Edward P. Feener, James G. Fujimoto

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Journal of Biomedical Optics, Vol. 22, Issue 1, 016005 (January 2017). https://doi.org/10.1117/1.JBO.22.1.016005

Abstract

The purpose of this study is to evaluate the suitability of five different anesthetic protocols (isoflurane, isoflurane–xylazine, pentobarbital, ketamine–xylazine, and ketamine–xylazine–vecuronium) for functional blood flow imaging in the rat eye. Total retinal blood flow was measured at a series of time points using an ultrahigh-speed Doppler OCT system. Additionally, each anesthetic protocol was qualitatively evaluated according to the following criteria: (1) time-stability of blood flow, (2) overall rate of blood flow, (3) ocular immobilization, and (4) simplicity. We observed that different anesthetic protocols produced markedly different blood flows. Different anesthetic protocols also varied with respect to the four evaluated criteria. These findings suggest that the choice of anesthetic protocol should be carefully considered when designing and interpreting functional blood flow studies in the rat eye.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Eric M. Moult, WooJhon Choi, David A. Boas, Bernhard Baumann, Allen C. Clermont, Edward P. Feener, and James G. Fujimoto "Evaluating anesthetic protocols for functional blood flow imaging in the rat eye," Journal of Biomedical Optics 22(1), 016005 (5 January 2017). https://doi.org/10.1117/1.JBO.22.1.016005

Received: 14 September 2016; Accepted: 15 December 2016; Published: 5 January 2017

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