Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination

Sanjee Abeytunge; Bjorg A. Larson; Gary Peterson; Monica Morrow; Milind Rajadhyaksha; Melissa P. Murray

doi:10.1117/1.JBO.22.3.034002

22 March 2017 Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination

Sanjee Abeytunge, Bjorg A. Larson, Gary Peterson, Monica Morrow, Milind Rajadhyaksha, Melissa P. Murray

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Journal of Biomedical Optics, Vol. 22, Issue 3, 034002 (March 2017). https://doi.org/10.1117/1.JBO.22.3.034002

Abstract

Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen- or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750×750 μm2. We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2×2 cm2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Sanjee Abeytunge, Bjorg A. Larson, Gary Peterson, Monica Morrow, Milind Rajadhyaksha, and Melissa P. Murray "Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination," Journal of Biomedical Optics 22(3), 034002 (22 March 2017). https://doi.org/10.1117/1.JBO.22.3.034002

Received: 31 October 2016; Accepted: 27 February 2017; Published: 22 March 2017

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