CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 23 > Issue 10 > Article
Open Access
1 October 2018 Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues
Bin Yang, Bryn Brazile, Ning-Jiun Jan, Yi Hua, Junchao Wei, Ian A. Sigal
Author Affiliations +
Journal of Biomedical Optics, Vol. 23, Issue 10, 106001 (October 2018). https://doi.org/10.1117/1.JBO.23.10.106001
Abstract
Collagen is a major constituent of the eye and understanding its architecture and biomechanics is critical to preserve and restore vision. We, recently, demonstrated polarized light microscopy (PLM) as a powerful technique for measuring properties of the collagen fibers of the eye, such as spatial distribution and orientation. Our implementation of PLM, however, required sectioning the tissues for imaging using transmitted light. This is problematic because it limits analysis to thin sections. This is not only slow, but precludes study of dynamic events such as pressure-induced deformations, which are central to the role of collagen. We introduce structured polarized light microscopy (SPLM), an imaging technique that combines structured light illumination with PLM to allow imaging and measurement of collagen fiber properties in thick ocular tissues. Using pig and sheep eyes, we show that SPLM rejects diffuse background light effectively in thick tissues, significantly enhancing visualization of optic nerve head (ONH) structures, such as the lamina cribrosa, and improving the accuracy of the collagen fiber orientation measurements. Further, we demonstrate the integration of SPLM with an inflation device to enable direct visualization, deformation tracking, and quantification of collagen fibers in ONHs while under controlled pressure.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2018/$25.00 © 2018 SPIE
Citation Download Citation
Bin Yang, Bryn Brazile, Ning-Jiun Jan, Yi Hua, Junchao Wei, and Ian A. Sigal "Structured polarized light microscopy for collagen fiber structure and orientation quantification in thick ocular tissues," Journal of Biomedical Optics 23(10), 106001 (1 October 2018). https://doi.org/10.1117/1.JBO.23.10.106001
Received: 2 July 2018; Accepted: 13 September 2018; Published: 1 October 2018
JOURNAL ARTICLE
 10 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 23 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Collagen
Tissues
Structured optical fibers
Microscopy
Polarized microscopy
Visualization
Optical tracking
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top