Two-wavelength oximetry of tissue microcirculation based on sidestream dark-field imaging

Ryohei Hashimoto; Tomohiro Kurata; Masashi Sekine; Kazuya Nakano; Takashi Ohnishi; Hideaki Haneishi

doi:10.1117/1.JBO.24.3.031013

30 October 2018 Two-wavelength oximetry of tissue microcirculation based on sidestream dark-field imaging

Ryohei Hashimoto, Tomohiro Kurata, Masashi Sekine, Kazuya Nakano, Takashi Ohnishi, Hideaki Haneishi

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Journal of Biomedical Optics, Vol. 24, Issue 3, 031013 (October 2018). https://doi.org/10.1117/1.JBO.24.3.031013

Abstract

Monitoring oxygen saturation (SO₂) in microcirculation is effective for understanding disease dynamics. We have developed an SO₂ estimation method, sidestream dark-field (SDF) oximetry, based on SDF imaging. SDF imaging is a noninvasive and clinically applicable technique to observe microcirculation. We report the first in vivo experiment observing the changes in SO₂ of microcirculation using SDF oximetry. First, heat from the light-emitting diodes used for the SDF imaging might affect hemodynamics in microcirculation, hence, we performed an experiment to evaluate the influence of that on the SDF oximetry. The result suggested that SDF oximetry had enough stability for long-term experiments. Then, to evaluate the sensitivity of SDF oximetry to alterations in the hemodynamics of the microcirculation, we observed the time-lapsed SO₂ changes in the dermis microcirculation of rats under hypoxic stimulation. We confirmed that the SO₂ estimated by SDF oximetry was in accordance with changes in the fraction of inspired oxygen (FiO₂). Thus, SDF oximetry is considered to be able to observe SO₂ changes that occur in accordance with alteration of the microcirculation.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Ryohei Hashimoto, Tomohiro Kurata, Masashi Sekine, Kazuya Nakano, Takashi Ohnishi, and Hideaki Haneishi "Two-wavelength oximetry of tissue microcirculation based on sidestream dark-field imaging," Journal of Biomedical Optics 24(3), 031013 (30 October 2018). https://doi.org/10.1117/1.JBO.24.3.031013

Received: 29 June 2018; Accepted: 10 October 2018; Published: 30 October 2018

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