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9 July 2019 Multi-spectral laser speckle contrast images using a wavelength-swept laser
Jeong Won Kim, Hansol Jang, Gyeong Hun Kim, Seung Won Jun, Chang-Seok Kim
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Journal of Biomedical Optics, Vol. 24, Issue 7, 076001 (July 2019). https://doi.org/10.1117/1.JBO.24.7.076001
Abstract
A multi-spectral laser speckle contrast imaging (MS-LSCI) system is proposed using only a single wavelength-swept laser, which provides both highly coherent and multi-spectral outputs to simultaneously generate laser speckle contrast images and multi-spectral images, respectively. Using a laser light swept from 770 to 821 nm at a repetition rate of 5 Hz and a CCD camera of 335 fps, 67 multi-spectral frame images are acquired in 0.76 nm wavebands over 51 nm spectral range. The spectral sub-windowing method of single wavelength-swept laser source is used to solve the lack of spectral information from a few individual light sources, which is a limitation of conventional MS-LSCI systems. In addition to the speckle flow index from the LSCI frames, the multi-spectrally encoded images can generate additional images of spectral absorbance. To further examine the performance of the MS-LSCI system, an in vivo cuff-induced ischemia experiment was conducted to show the real-time imaging of hemodynamic and blood oxygen saturation changes simultaneously over the entire 2.5  cm  ×  4.5  cm field of view.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Jeong Won Kim, Hansol Jang, Gyeong Hun Kim, Seung Won Jun, and Chang-Seok Kim "Multi-spectral laser speckle contrast images using a wavelength-swept laser," Journal of Biomedical Optics 24(7), 076001 (9 July 2019). https://doi.org/10.1117/1.JBO.24.7.076001
Received: 11 December 2018; Accepted: 6 June 2019; Published: 9 July 2019
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Cited by 3 scholarly publications.
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KEYWORDS
Speckle
Absorbance
Imaging systems
Laser speckle contrast imaging
Absorption
Signal to noise ratio
Cameras
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