Detecting lipid metabolism responses in breast cancer under insulin and methionine regulation using Raman scattering and TPF microscopy

Anthony A. Fung; Lingyan Shi; Khang Hoang

doi:10.1117/12.2610592

2 March 2022 Detecting lipid metabolism responses in breast cancer under insulin and methionine regulation using Raman scattering and TPF microscopy

Anthony A. Fung, Lingyan Shi, Khang Hoang

Author Affiliations +

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119731A (2022) https://doi.org/10.1117/12.2610592
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Two breast cancer subtypes with contrasting aggressiveness and prognoses - triple negative and normal-like - had their lipid droplets interrogated under methionine and insulin manipulation. Raman scattering results suggest that excess methionine increases droplet volume and de novo lipogenesis but decreases droplet number and alters distribution in both subtypes. These effects are significantly augmented in the triple-negative subtype with the presence of insulin. Flavin autofluorescence increases with insulin but decreases with excess methionine, in which an abundance of unsaturated lipids was also observed. This suggests interplay between methionine and insulin related lipid homeostasis and demonstrates the utility of optical techniques.

Conference Presentation

Citation Download Citation

Anthony A. Fung, Lingyan Shi, and Khang Hoang "Detecting lipid metabolism responses in breast cancer under insulin and methionine regulation using Raman scattering and TPF microscopy", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119731A (2 March 2022); https://doi.org/10.1117/12.2610592

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