PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
We report a two-photon excitation fluorescence microscopy imager that uses a fiber-delivered fs supercontinuum (SC) generation source to achieve simultaneous dual-wavelength imaging. The SC generation is based on pumping a polarization-maintaining photonic crystal fiber with a fiber laser. The SC is then delivered to the imaging head through a broadband hollow-core fiber. Excitation wavelengths centered at 920 nm and 1180 nm were selected, which enabled simultaneous imaging of neurons transfected with GCaMP6 and administrated with muscimol-conjugated BODIPY. Imaging results of calcium dynamics of in vivo mouse brain and cultured primary neurons were collected to demonstrate the technology feasibility.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.