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15 March 2023 Construction and validation of a multimodal 3D single-molecule super-resolution microscope for whole cell imaging (Conference Presentation)
Tyler Nelson, Sofia Vargas-Hernandez, Ivana Hsyung, Margareth Freire, Anna-Karin Gustavsson
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Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC123860D (2023) https://doi.org/10.1117/12.2649495
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The appropriate sample illumination method varies depending on the nature of the sample and the goals of the study, especially for single-molecule localization microscopy. Here, we demonstrate a flexible microscopy system which combines the whole-cell sectioning capability of light sheet with conventional epi-illumination and total internal reflection fluorescence (TIRF), both with a homogeneous flat-field profile to significantly improve the resolution across the entire field of view. In conjunction with point spread function engineering capabilities and fast switching of illumination modes, our system enables 3D single-molecule super-resolution imaging optimized for the sample and region of interest.
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Tyler Nelson, Sofia Vargas-Hernandez, Ivana Hsyung, Margareth Freire, and Anna-Karin Gustavsson "Construction and validation of a multimodal 3D single-molecule super-resolution microscope for whole cell imaging (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC123860D (15 March 2023); https://doi.org/10.1117/12.2649495
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KEYWORDS
3D image processing
Microscopes
Super resolution
Imaging systems
Luminescence
Molecules
Point spread functions
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