New approach to double-helix point spread function design for 3D super-resolution microscopy

Ginni Grover; Rafael Piestun

doi:10.1117/12.2005694

22 February 2013 New approach to double-helix point spread function design for 3D super-resolution microscopy

Ginni Grover, Rafael Piestun

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 85900M (2013) https://doi.org/10.1117/12.2005694
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Double-helix point spread functions (DH-PSFs) have been used to extend localization and super-resolution microscopy to three dimensions. Current DH-PSF design provides a long depth-of-field for 3D imaging. However, it is not optimal for imaging under high background conditions. We present a method to design unconventional DH-PSFs with control on characteristics such as efficiency, transverse spread, and Fisher information. This allows tailoring the PSFs for specific applications. In particular, we introduce a design suitable for 3D localization-based super-resolution imaging under typical background conditions and demonstrate imaging with resolution one order of magnitude beyond the diffraction limit over a depth-of-field of 1.2 μm.

Citation Download Citation

Ginni Grover and Rafael Piestun "New approach to double-helix point spread function design for 3D super-resolution microscopy", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900M (22 February 2013); https://doi.org/10.1117/12.2005694

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