In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

Viacheslav Mazlin; Peng Xiao; Eugénie Dalimier; Kate Grieve; Kristina Irsch; José Sahel M.D.; Mathias Fink; Claude Boccara

doi:10.1117/12.2288947

19 February 2018 In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography

Viacheslav Mazlin, Peng Xiao, Eugénie Dalimier, Kate Grieve, Kristina Irsch, José Sahel M.D., Mathias Fink, Claude Boccara

Author Affiliations +

Proceedings Volume 10474, Ophthalmic Technologies XXVIII; 104740S (2018) https://doi.org/10.1117/12.2288947
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Despite obvious improvements in visualization of the in vivo cornea through the faster imaging speeds and higher axial resolutions, cellular imaging stays unresolvable task for OCT, as en face viewing with a high lateral resolution is required. The latter is possible with FFOCT, a method that relies on a camera, moderate numerical aperture (NA) objectives and an incoherent light source to provide en face images with a micrometer-level resolution. Recently, we for the first time demonstrated the ability of FFOCT to capture images from the in vivo human cornea1. In the current paper we present an extensive study of appearance of healthy in vivo human corneas under FFOCT examination. En face corneal images with a micrometer-level resolution were obtained from the three healthy subjects. For each subject it was possible to acquire images through the entire corneal depth and visualize the epithelium structures, Bowman’s layer, sub-basal nerve plexus (SNP) fibers, anterior, middle and posterior stroma, endothelial cells with nuclei. Dimensions and densities of the structures visible with FFOCT, are in agreement with those seen by other cornea imaging methods. Cellular-level details in the images obtained together with the relatively large field-of-view (FOV) and contactless way of imaging make this device a promising candidate for becoming a new tool in ophthalmological diagnostics.

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Viacheslav Mazlin, Peng Xiao, Eugénie Dalimier, Kate Grieve, Kristina Irsch, José Sahel M.D., Mathias Fink, and Claude Boccara "In vivo imaging through the entire thickness of human cornea by full-field optical coherence tomography", Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104740S (19 February 2018); https://doi.org/10.1117/12.2288947

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KEYWORDS

Cornea

In vivo imaging

Optical coherence tomography

Image resolution

Cameras

Eye

Imaging devices

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