Kassandra Groux,1,2 Jules Scholler,3 Pedro Mecê,3 Sacha Reichman,4 Valérie Fradot,4 Michel Pâques,4 Claude Boccara,3 Katharine Grievehttps://orcid.org/0000-0002-2937-62854
1Institut Langevin Ondes et Images, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris (France) 2Univ. PSL, CNRS (France) 3Institut Langevin Ondes et Images (France) 4Institut de la Vision, Ctr. Hospitalier National d'Opthalmologie des Quinze-Vingts (France)
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Dynamic FFOCT allows us to see the sub-cellular motion of biological samples. We are able to follow the evolution in the same plane of biological samples for hours thanks to an autofocus procedure. RPE cells are involved in the integrity of retina and vision. We performed a linear scratch assay in RPE cell culture with a surgical scalpel blade, inducing border cell migration at 20.8 µm/h to close the scratch. We also recorded motility of microvilli, thanks to rapid GPU computing. Quantitative live imaging of RPE cell culture with DFFOCT is useful in development of disease models of retinal degeneration.
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Kassandra Groux, Jules Scholler, Pedro Mecê, Sacha Reichman, Valérie Fradot, Michel Pâques, Claude Boccara, Katharine Grieve, "Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)," Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390O (9 March 2020); https://doi.org/10.1117/12.2544653