Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)

Kassandra Groux; Jules Scholler; Pedro Mecê; Sacha Reichman; Valérie Fradot; Michel Pâques; Claude Boccara; Katharine Grieve

doi:10.1117/12.2544653

9 March 2020 Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)

Kassandra Groux, Jules Scholler, Pedro Mecê, Sacha Reichman, Valérie Fradot, Michel Pâques, Claude Boccara, Katharine Grieve

Author Affiliations +

Proceedings Volume 11239, Dynamics and Fluctuations in Biomedical Photonics XVII; 112390O (2020) https://doi.org/10.1117/12.2544653
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Dynamic FFOCT allows us to see the sub-cellular motion of biological samples. We are able to follow the evolution in the same plane of biological samples for hours thanks to an autofocus procedure. RPE cells are involved in the integrity of retina and vision. We performed a linear scratch assay in RPE cell culture with a surgical scalpel blade, inducing border cell migration at 20.8 µm/h to close the scratch. We also recorded motility of microvilli, thanks to rapid GPU computing. Quantitative live imaging of RPE cell culture with DFFOCT is useful in development of disease models of retinal degeneration.

Conference Presentation

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Kassandra Groux, Jules Scholler, Pedro Mecê, Sacha Reichman, Valérie Fradot, Michel Pâques, Claude Boccara, and Katharine Grieve "Stress and repair in retinal pigmented epithelium cell culture imaged with dynamic full-field OCT (Conference Presentation)", Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390O (9 March 2020); https://doi.org/10.1117/12.2544653

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