High-speed label-free galvo mirror scanning-based ultraviolet photoacoustic microscopy for histological imaging

Xiufeng Li; Lei Kang; Yan Zhang; Terence T. W. Wong

doi:10.1117/12.2544071

17 February 2020 High-speed label-free galvo mirror scanning-based ultraviolet photoacoustic microscopy for histological imaging

Xiufeng Li, Lei Kang, Yan Zhang, Terence T. W. Wong

Author Affiliations +

Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112401X (2020) https://doi.org/10.1117/12.2544071
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Ultraviolet-based photoacoustic microscopy (UV-PAM) has recently been demonstrated as a promising tool to overcome the time-consuming sample preparation procedure in traditional pathological analysis. In order to achieve high-speed UVPAM for clinical usage, we implemented UV-PAM with a single-axis galvo mirror scanner. With our UV laser operating at a repetition rate of 55 kHz, our system produced images ~5.5 times faster than the previously reported point-by-point raster scanning based UV-PAM, with a lateral resolution of ~1.0 μm. Histology-like images of a mouse brain slice were acquired by our system, showing its potential as an intraoperative imaging tool for surgical margin assessment.

Conference Presentation

Citation Download Citation

Xiufeng Li, Lei Kang, Yan Zhang, and Terence T. W. Wong "High-speed label-free galvo mirror scanning-based ultraviolet photoacoustic microscopy for histological imaging", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112401X (17 February 2020); https://doi.org/10.1117/12.2544071

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