Multiline illumination Raman microscopy for rapid cell imaging

Kentaro Mochizuki; Yasuaki Kumamoto; Katsumasa Fujita

doi:10.1117/12.2573222

15 June 2020 Multiline illumination Raman microscopy for rapid cell imaging

Kentaro Mochizuki, Yasuaki Kumamoto, Katsumasa Fujita

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 115210F (2020) https://doi.org/10.1117/12.2573222
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

Acceleration of image acquisition rate in Raman microscopy has been required to fully utilize its analytical advantages for biological/medical applications. By introducing the multiple line illumination and parallel spectral detection capability, image acquisition rate in the Raman microscope was improved < 104 times, compared with the conventional confocal Raman. High-resolution spontaneous Raman imaging of cells/tissues was thus enabled with an image acquisition time of a few minutes. Subsequent high-throughput Raman imaging-based analyses were also performed, including multiplex Raman tag imaging, cell classification, microplastic detection.

Citation Download Citation

Kentaro Mochizuki, Yasuaki Kumamoto, and Katsumasa Fujita "Multiline illumination Raman microscopy for rapid cell imaging", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210F (15 June 2020); https://doi.org/10.1117/12.2573222

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