Variation of optical attenuation coefficient during ischemia-reperfusion process of rabbit kidney measured by optical coherence tomography

Yuhong Fang; Wei Gong; Zheng Huang; Tao Li; Xiang Wu; Qingjiang Xu; Deyuan Yang; Shusen Xie

doi:10.1117/12.2575207

10 October 2020 Variation of optical attenuation coefficient during ischemia-reperfusion process of rabbit kidney measured by optical coherence tomography

Yuhong Fang, Wei Gong, Zheng Huang, Tao Li, Xiang Wu, Qingjiang Xu, Deyuan Yang, Shusen Xie

Author Affiliations +

Proceedings Volume 11553, Optics in Health Care and Biomedical Optics X; 115532R (2020) https://doi.org/10.1117/12.2575207
Event: SPIE/COS Photonics Asia, 2020, Online Only

Conference Poster

Abstract

Optical properties of biological tissue are key parameters of optical imaging, and also provide useful information of the tissues under different physiological conditions. The optical attenuation coefficient (OAC) related to the optical properties can be calculated from optical coherence tomography(OCT) data. OCT has the advantages of high-resolution and fast imaging speed, and can image the tissues in vivo and in real-time. Due to the lack of blood perfusion, renal ischemia is accompanied by changes in microstructure of the kidney, which the OAC is sensitive to. We applied OCT to detect the OAC variation during ischemia-reperfusion process of rabbit kidney, and further estimated the ischemiareperfusion (I/R) injury. In order to study the temporal relationship between I/R injury and ischemia, 12 New Zealand rabbits were divided averagely into 4 groups (ischemia 30/60/90/120 min group). The kidneys were observed in vivo using a spectral domain OCT (SD-OCT) which light source centered at 900 nm. Three-dimensional OCT images of the kidney were obtained before the occlusion of renal artery and several time points after the blood reperfusion. The OAC were obtained by exponential fitting of OCT A-lines. Mapping attenuation coefficient (MAC) of each 3D OCT data set was performed to get the attenuation coefficient distribution in the kidney. The OAC curve with reperfusion time showed that the OAC was sensitive to ischemia and helpful for the estimation of ischemia-reperfusion injury. The distribution of attenuation coefficient in MAC image could reflect the local status of kidney.

Citation Download Citation

Yuhong Fang, Wei Gong, Zheng Huang, Tao Li, Xiang Wu, Qingjiang Xu, Deyuan Yang, and Shusen Xie "Variation of optical attenuation coefficient during ischemia-reperfusion process of rabbit kidney measured by optical coherence tomography", Proc. SPIE 11553, Optics in Health Care and Biomedical Optics X, 115532R (10 October 2020); https://doi.org/10.1117/12.2575207

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available