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3D quantitative phase imaging (3D QPI) delivers volumetric information about the refractive index distribution within microobjects. Such imaging and measurement capabilities are of great interest for technical and biomedical applications, yet there are no standardized methods for testing and reporting metrological performance. In this paper we present methodology for metrological evaluation of instruments based on single wavelength and hyperspectral holographic tomography in biomedical applications. The methodology entails suitable phantoms, quality assessment metric and easily reproducible protocol that is attainable for both numerical and experimental analysis. We demonstrate its applicability by comparing simulated reconstructions with measurements from 3 holographic tomography systems.
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Malgorzata Kujawinska, Michal Ziemczonok, "Metrological assessment of reconstruction accuracy in holographic tomography (Conference Presentation)," Proc. SPIE 12223, Interferometry XXI, 122230D (3 October 2022); https://doi.org/10.1117/12.2633939