Higher density of ECM composition in pancreatic cancer correlates with reduced drug delivery

Cody C. Rounds; Sanzida Haque; Chengyue Li; Devin Nissen; Dhruv Ranjan; Marcella Vaicik; Rama S. Madhurapantula; Thomas C. Irving; Kenneth M. Tichauer

doi:10.1117/12.2650840

14 March 2023 Higher density of ECM composition in pancreatic cancer correlates with reduced drug delivery

Cody C. Rounds, Sanzida Haque, Chengyue Li, Devin Nissen, Dhruv Ranjan, Marcella Vaicik, Rama S. Madhurapantula, Thomas C. Irving, Kenneth M. Tichauer

Author Affiliations +

Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 123570B (2023) https://doi.org/10.1117/12.2650840
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Pancreatic ductal adenocarcinomas (PDACs) are often treatment resistant, and as such widefield imaging methods for the evaluation of ECM composition are needed. Here we present a method to measure the relative abundance of ECM diffracting components in PDAC samples alongside drug penetration in widefield images. Orthotopic mouse PDAC xenografts are grown and assessment of drug penetration as well as ECM composition is done using co-registration of scanning x-ray diffraction (XRD) and EGFR-specific drug penetration fluorescent widefield images. Preliminary data suggests a strongly negative correlation between abundance of diffracting ECM components and penetration of large drugs in solid tumors. This methodology may be used to provide crucial insights into both drug-development approaches and multi-therapeutic treatment strategies in late stage PDAC patients presenting with ECM desmoplasia.

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Citation Download Citation

Cody C. Rounds, Sanzida Haque, Chengyue Li, Devin Nissen, Dhruv Ranjan, Marcella Vaicik, Rama S. Madhurapantula, Thomas C. Irving, and Kenneth M. Tichauer "Higher density of ECM composition in pancreatic cancer correlates with reduced drug delivery", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 123570B (14 March 2023); https://doi.org/10.1117/12.2650840

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