Two-photon excitation microscopy through scattering media using digital optical phase conjugation (DOPC)

Seungmin Lee; Mooseok Jang

doi:10.1117/12.3017924

26 February 2024 Two-photon excitation microscopy through scattering media using digital optical phase conjugation (DOPC)

Seungmin Lee, Mooseok Jang

Author Affiliations +

Proceedings Volume 13076, SPIE Advanced Biophotonics Conference (SPIE ABC 2023); 1307607 (2024) https://doi.org/10.1117/12.3017924
Event: SPIE Advanced Biophotonics Conference (SPIE ABC 2023), 2023, Jeju, Korea, Republic of

Abstract

Multiphoton microscopy, especially two-photon microscopy has become a gold-standard technique for in vivo biological tissue imaging, specifically for neural activity recording. However, due to the heterogeneity of the biological tissue, the excitation beam will undergo wavefront distortion and temporal broadening while passing through the scattering layer such as the skull and cortex. To overcome this challenge, wavefront shaping has been explored. Iterative modulation of the wavefront modulator using indirect wavefront measurement or transmission matrix approaches in a close-looped feedback system is widely used to compensate the effect of scattering for the ultrashort pulse laser source. However, these techniques, entailing multiple manipulations and calibrations for optimization, are time-intensive and restrictive in achieving high temporal resolution for simultaneous neural recording. In this research, by applying a ‘time-gating’ technique to digital optical phase conjugation (DOPC), or time-reversal, we can make an optimal wavefront to make a focus, both spatially and temporally. Consequently, it proposes the potential of this approach for non-invasive deep tissue neural recording in vivo. Since this process is open-looped system, this method is much faster than conventional feedback-based optimization and suitable for application in simultaneous neural recording.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Seungmin Lee and Mooseok Jang "Two-photon excitation microscopy through scattering media using digital optical phase conjugation (DOPC)", Proc. SPIE 13076, SPIE Advanced Biophotonics Conference (SPIE ABC 2023), 1307607 (26 February 2024); https://doi.org/10.1117/12.3017924

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Wavefronts

Pulse signals

In vivo imaging

Phase conjugation

Laser scattering

Two photon excitation microscopy

Fluorescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years