Study on the optimizing determination of selenocysteine content based on fluorescence method

Yan Gao; Linlin Bian; Xiaofang Ma; Wen He; Hui Wang; Li Han; Chaoyue Shan; Yunhao Huang; Zhicheng Yu

doi:10.1117/12.3032355

19 June 2024 Study on the optimizing determination of selenocysteine content based on fluorescence method

Yan Gao, Linlin Bian, Xiaofang Ma, Wen He, Hui Wang, Li Han, Chaoyue Shan, Yunhao Huang, Zhicheng Yu

Proceedings Volume 13172, Ninth International Symposium on Energy Science and Chemical Engineering (ISESCE 2024) ; 131720V (2024) https://doi.org/10.1117/12.3032355
Event: 9th International Symposium on Energy Science and Chemical Engineering, 2024, Nanjing, China

Abstract

The determination of selenocysteine was investigated based on the fact that the -SeH bond in selenols can specifically undergo nucleophilic addition reaction with the Se-N bond in benzoselenadiazole. The results obtained showed that the pH of t determination system was 4.7 and the optimum temperature was 37°C. The linear relationship was F=90.246c+298.36, and the correlation coefficient R² was 0.9916. The relative error of this method was less than 2.2%, and the recoveries were in the range of 96.6%~103%. This experimental method is based on the nucleophilic addition reaction of -SeH, which enables the rapid and accurate determination of selenocysteine without catalysis, heating and long waiting time. This approach provides some reference for the study of the physiological significance of selenium.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yan Gao, Linlin Bian, Xiaofang Ma, Wen He, Hui Wang, Li Han, Chaoyue Shan, Yunhao Huang, and Zhicheng Yu "Study on the optimizing determination of selenocysteine content based on fluorescence method", Proc. SPIE 13172, Ninth International Symposium on Energy Science and Chemical Engineering (ISESCE 2024) , 131720V (19 June 2024); https://doi.org/10.1117/12.3032355

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