Photodetection of early cancer in the upper aerodigestive tract and the bronchi using photofrin II and colorectal adenocarcinoma with fluoresceinated monoclonal antibodies

Georges A. Wagnieres; Daniel Braichotte; Andre Chatelain; Christian D. Depeursinge; Philippe Monnier; Jean-Francois Savary; Charlotte Fontolliet; J.-M. Calmes; Jean-Claude Givel; G. Chapuis; S. Folli; A. Pelegrin; F. Buchegger; J.-P. Mach; Hubert van den Bergh

doi:10.1117/12.48224

1 November 1991 Photodetection of early cancer in the upper aerodigestive tract and the bronchi using photofrin II and colorectal adenocarcinoma with fluoresceinated monoclonal antibodies

Georges A. Wagnieres, Daniel Braichotte, Andre Chatelain, Christian D. Depeursinge, Philippe Monnier, Jean-Francois Savary, Charlotte Fontolliet, J.-M. Calmes, Jean-Claude Givel, G. Chapuis, S. Folli, A. Pelegrin, F. Buchegger, J.-P. Mach, Hubert van den Bergh

Author Affiliations +

Proceedings Volume 1525, Future Trends in Biomedical Applications of Lasers; (1991) https://doi.org/10.1117/12.48224
Event: MedTech '91, 1991, Berlin, Germany

Abstract

The performance of a fluorescence endoscope for the detection of early cancer is clinically evaluated. the apparatus is based on the imaging of the laser-induced fluorescence (LIF) of a dye which localizes in the tumor after IV injection with a higher concentration than in the surrounding normal tissue. The tests are carried out in several of the hollow organs, such as the upper aerodigestive tract, the bronchi, and the colon. In the two former cases the dye used is photofrin II, whereas in the latter case conjugates between monoclonal antibodies (Mab) directed against carcinoembrionic antigen (CEA) and fluorescein molecules are injected. The fluorescence contrast between tumor and surrounding tissue is enhanced by real-time image processing which eliminates most of the tissue autofluorescence as well as the fluorescence due to the relatively small amount of dye localized in the normal tissue. This is done by recording the fluorescence image in two spectral domains, after which these two images are digitized and manipulated with a mathematical operator (lookup-table). The sources of false positives and false negatives are evaluated in terms of the fluorescent dye and tissue optical properties.

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Georges A. Wagnieres, Daniel Braichotte, Andre Chatelain, Christian D. Depeursinge, Philippe Monnier, Jean-Francois Savary, Charlotte Fontolliet, J.-M. Calmes, Jean-Claude Givel, G. Chapuis, S. Folli, A. Pelegrin, F. Buchegger, J.-P. Mach, and Hubert van den Bergh "Photodetection of early cancer in the upper aerodigestive tract and the bronchi using photofrin II and colorectal adenocarcinoma with fluoresceinated monoclonal antibodies", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); https://doi.org/10.1117/12.48224

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