Frozen-solution MALDI mass spectrometry studies of DNA

Joanna M. Hunter; Hua Lin; Kristin A. Sannes; Christopher H. Becker

doi:10.1117/12.237627

1 April 1996 Frozen-solution MALDI mass spectrometry studies of DNA

Joanna M. Hunter, Hua Lin, Kristin A. Sannes, Christopher H. Becker

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237627
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Matrix-assisted laser desorption/ionization (MALDI) in conjunction with time-of-flight mass spectrometry is an effective technique for analysis of DNA oligomers. However, with increasing oligomer length, mass resolution and sensitivity degrade as the effects of prompt and metastable decay, adduct formation, and ion kinetic energy spreads become increasingly dominant. Attempts to reduce these factors have focused on the use of volatile liquids as matrices because these molecules have lower binding energies both to themselves and to the DNA and theoretically require less energy for desorption. We have investigated thin films of DNA in liquid matrices frozen on a liquid nitrogen-cooled sample stage. Several solvent systems have resulted in reproducible mass spectra of single-stranded DNA oligomers.

Citation Download Citation

Joanna M. Hunter, Hua Lin, Kristin A. Sannes, and Christopher H. Becker "Frozen-solution MALDI mass spectrometry studies of DNA", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237627

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