New optical configuration for flow cytometric sorting of aspherical cells

John C. Sharpe; Peter N. Schaare; Rainer Kuennemeyer

doi:10.1117/12.273633

2 May 1997 New optical configuration for flow cytometric sorting of aspherical cells

John C. Sharpe, Peter N. Schaare, Rainer Kuennemeyer

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273633
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The orthogonal axes of illumination, flow, and detection in conventional sorting flow cytometers can limit accuracy or throughput when making fluorescence measurements on a spherical cells. A new radially symmetric optical configuration has been designed to overcome these problems. Both illumination and fluorescence collection are performed by a single optical element which encircles the sample stream flow axis. Unlike existing epi-illumination flow cytometer designs, these optics are compatible with electrostatic sorting. The resolution of this system is currently being evaluated for DNA chromosome content measurement with an ultimate goal of separation of X- and Y- chromosome-bearing mammalian spermatozoa. We describe the new optical configuration and present preliminary results of instrument performance. Comparison with a conventional orthogonal optical geometry is made using fluorescent microspheres, chicken red blood cells and chinchilla sperm.

Citation Download Citation

John C. Sharpe, Peter N. Schaare, and Rainer Kuennemeyer "New optical configuration for flow cytometric sorting of aspherical cells", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273633

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