Modification of tumor response by manipulation of tumor oxygenation

Qun Chen; Jill Beckers; Fred W. Hetzel

doi:10.1117/12.351506

6 July 1999 Modification of tumor response by manipulation of tumor oxygenation

Qun Chen, Jill Beckers, Fred W. Hetzel

Proceedings Volume 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII; (1999) https://doi.org/10.1117/12.351506
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Photodynamic therapy (PDT) requires tissue oxygenation during light irradiation. Tumor hypoxia, either pre-existing or induced by PDT during light irradiation, can severely hamper the effectiveness of a PDT treatment. Lowering the light irradiation does rate or fractionating a light dose may improve cell kill of PDT induced hypoxic cells, but will have no effects on pre-existing hypoxic cells. In the current study, we used hyper-oxygenation during PDT to overcome cell hypoxia in PDT. C3H mice with transplanted mammary carcinoma tumor were injected with 12.5 mg/kg Photofrin and irradiated with 630 nm laser light 24 hours later. Tumor oxygenation was manipulated by subjecting the animals to 3 a.t.p. hyperbaric oxygen or normobaric oxygen during PDT light irradiation. The results show a significant improvement in tumor response when PDT was delivered during hyper-oxygenation. With hyper-oxygenation, up to 80% of treated tumors showed no re-growth after 60 days. In comparison, only 20% of tumors treated while animals breathed normal room air, did not re-grow. To quantitatively evaluate the effects of manipulating tumor oxygenation, tumor p02 was measured with microelectrodes positioned in pre-existing hypoxic regions before and during the PDT light irradiation. The results show that hyper-oxygenation may oxygenate pre-existing hypoxic cells and compensate oxygen depletion induced by PDT light irradiation. In conclusion, hyper-oxygenation may provide effective ways to improve PDT treatment efficiency by oxygenating both pre-existing and treatment induced cell hypoxia.

Citation Download Citation

Qun Chen, Jill Beckers, and Fred W. Hetzel "Modification of tumor response by manipulation of tumor oxygenation", Proc. SPIE 3592, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy VIII, (6 July 1999); https://doi.org/10.1117/12.351506

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KEYWORDS

Tumors

Photodynamic therapy

Oxygen

Tissues

Hypoxia

Tissue optics

Animal model studies

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