Laser-induced microscopic fluorescence and images of skin tissues

Zhiwei Huang; Horn Mun Cheah; Teck-Chee Chia; Tan Li Ching

doi:10.1117/12.410627

18 December 2000 Laser-induced microscopic fluorescence and images of skin tissues

Zhiwei Huang, Horn Mun Cheah, Teck-Chee Chia, Tan Li Ching

Proceedings Volume 4164, Laser Microscopy; (2000) https://doi.org/10.1117/12.410627
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The microscopic autofluorescence and images of different tissue layer in normal and cancerous skin tissues were studied under a micro spectrophotometric system when using a laser light at 442 nm. The results show that the autofluorescence images of normal skin tissues are much brighter than the cancerous ones, and the autofluorescence intensity from various tissue layers of normal skin is stronger than that of cancerous skin tissues. It is also found that the dermis emits more intense fluorescence while epidermis fluoresces weakly in the skin tissues. The obtained data on the microscopic fluorescence properties in the skin will be useful to aid in the interpretation of underlying mechanisms by which the laser-induced autofluorescence technique differentiates between normal and cancerous skin tissues.

Citation Download Citation

Zhiwei Huang, Horn Mun Cheah, Teck-Chee Chia, and Tan Li Ching "Laser-induced microscopic fluorescence and images of skin tissues", Proc. SPIE 4164, Laser Microscopy, (18 December 2000); https://doi.org/10.1117/12.410627

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available