Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer

Tongsheng Chen; Shaoqun Zeng; Qingming Luo; Zhihong Zhang; Wei Zhou

doi:10.1117/12.403990

11 October 2000 Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer

Tongsheng Chen, Shaoqun Zeng, Qingming Luo, Zhihong Zhang, Wei Zhou

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403990
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

Green fluorescent protein based fluorescence resonance energy transfer is widely used in studies of inter-and intra-molecular dynamic interactions in alive cells. But it is a trade off between quantitative measurement and real time measurement because of the emission cross talk. In stead of using more than one excitation wavelength and several sets of filters, we proposed a more efficient correction scheme which needs only a single excitation and one filter set. This method makes use of the known emission spectra of the fluorophores and can be run in real time. The validity and advantages were demonstrated by experiments with chamaeleons.

Citation Download Citation

Tongsheng Chen, Shaoqun Zeng, Qingming Luo, Zhihong Zhang, and Wei Zhou "Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403990

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available