Autofluorescence diagnostic algorithm for detecting malignant colonic tissues

Zhiwei Huang; Wei Zheng; Teck-Chee Chia; Cheong Hoong Diong; Tuan-Kay Lim; Francis Choen Seow; Haishan Zeng; Calum E. MacAulay; D. I. Maclean; Harvey Lui M.D.

doi:10.1117/12.446650

25 October 2001 Autofluorescence diagnostic algorithm for detecting malignant colonic tissues

Zhiwei Huang, Wei Zheng, Teck-Chee Chia, Cheong Hoong Diong, Tuan-Kay Lim, Francis Choen Seow, Haishan Zeng, Calum E. MacAulay, D. I. Maclean, Harvey Lui M.D.

Author Affiliations +

Proceedings Volume 4597, Biophotonics Instrumentation and Analysis; (2001) https://doi.org/10.1117/12.446650
Event: International Symposium on Photonics and Applications, 2001, Singapore, Singapore

Abstract

The ratio of autofluorescence intensity at 550+/- 10 nm to that at 630+/- 10 nm (R₁=I₅₅₀/I₆₃₀) or 680+/- 10 nm (R₂=I₅₅₀/I₆₈₀) was used as a diagnostic algorithm for identification of malignant tumour tissues. The performance of the ratio diagnostic algorithm was evaluated on more than 100 human colonic specimens under excitation laser light at 457.9 nm. A significant difference of the ratio value R₁ and R₂ was found between normal and tumour specimens (p<0.001). The diagnostic test has a sensitivity and specificity of 85% and 87% for a threshold value of R₁=1.75, and a sensitivity and specificity of 94% and 82% for a threshold of R₂=3.5. The experimental results show that the ratio mapping is sensitive to the small changes in the presence of tumour tissues, and the contrast of the contour map for detecting the region of malignancy can also be enhanced significantly if using the diagnostic algorithm R₂ (I₅₅₀/I₆₈₀).

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Zhiwei Huang, Wei Zheng, Teck-Chee Chia, Cheong Hoong Diong, Tuan-Kay Lim, Francis Choen Seow, Haishan Zeng, Calum E. MacAulay, D. I. Maclean, and Harvey Lui M.D. "Autofluorescence diagnostic algorithm for detecting malignant colonic tissues", Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); https://doi.org/10.1117/12.446650

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