Effects of the bile acid UDCA on PDT efficacy in vitro and in vivo

David Kessel; Michelle Castelli; Elizabeth Sykes M.D.; Greta M. Garbo

doi:10.1117/12.524539

14 June 2004 Effects of the bile acid UDCA on PDT efficacy in vitro and in vivo

David Kessel, Michelle Castelli, Elizabeth Sykes M.D., Greta M. Garbo

Proceedings Volume 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII; (2004) https://doi.org/10.1117/12.524539
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The phototoxicity of PDT in cell culture can be promoted by the relatively hydrophilic bile acid UDCA (ursodeoxycholic acid). This was attributed to a conformational change in the anti-apoptotic protein Bcl-2, leading to an enhanced sensitivity to photodamage by sensitizers that target sites of Bcl-2 localization. UDCA also promoted the binding and inactivation of Bcl-2 by the non-peptidic antagonist HA14- 1, suggesting that UDCA may also be useful for promoting chemotherapy designed to target Bcl-2. In tumor-bearing animals, addition of UDCA to a PDT protocol involving the tin etiopurpurin SnET2 resulted in enhanced cancer control, but there was no effect on the extent of PDT-induced vascular shut-down. These results are consistent with the propo proposal that UDCA only promotes direct tumor cell kill. In this report, we have sal summarized recent research relating to mode of action of UDCA as it effects the on the efficacy of photodynamic therapy where Bcl-2 is among the PDT targets, and discuss the implications of the results.

Citation Download Citation

David Kessel, Michelle Castelli, Elizabeth Sykes M.D., and Greta M. Garbo "Effects of the bile acid UDCA on PDT efficacy in vitro and in vivo", Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); https://doi.org/10.1117/12.524539

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