Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions

Lixin Liu; Ziyang Lin; Junle Qu; Danni Chen; Gaixia Xu; Baoping Guo; Hanben Niu

doi:10.1117/12.710079

27 October 2006 Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions

Lixin Liu, Ziyang Lin, Junle Qu, Danni Chen, Gaixia Xu, Baoping Guo, Hanben Niu

Author Affiliations +

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472T (2006) https://doi.org/10.1117/12.710079
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Multifocal multiphoton microscopy (MMM) is a more efficient and powerful method for three-dimensional (3-D) fluorescence imaging with reduced acquisition time compared with conventional confocal and two-photon excitation fluorescence microscopy. We present a novel multifocal two-photon excitation fluorescence sampling imaging technique that is based on a specially designed streak camera and combines fluorescence lifetime and spectrum resolutions. A proof-of-principle experiment is performed on a standard fluorescent dye solution (Rhodamine 6G in ethanol), Time- and spectrum-resolved sampled fluorescence image of Rhodamine 6G is obtained in a snapshot. The reconstructed two-dimensional (2-D) fluorescence image of a prepared plant slide is also obtained by moving the sample laterally. The capability of this system capable of performing simultaneous 2-D measurements of temporal and spectral information has many potential applications, e.g., multi-well imaging and spectrally resolved multifocal multiphoton fluorescence lifetime imaging etc.

Citation Download Citation

Lixin Liu, Ziyang Lin, Junle Qu, Danni Chen, Gaixia Xu, Baoping Guo, and Hanben Niu "Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472T (27 October 2006); https://doi.org/10.1117/12.710079

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available