Five-dimensional multifocal multiphoton microscopy

Lixin Liu; Junle Qu D.D.S.; Lei Wang; Ziyang Lin; Zhe Fu; Baoping Guo; Wenqing Liu; Hanben Niu

doi:10.1117/12.741580

1 May 2007 Five-dimensional multifocal multiphoton microscopy

Lixin Liu, Junle Qu D.D.S., Lei Wang, Ziyang Lin, Zhe Fu, Baoping Guo, Wenqing Liu, Hanben Niu

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341Q (2007) https://doi.org/10.1117/12.741580
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

We present a novel 5-dimensional (5-D) MMM system that is based on a microlens array for producing discrete excitation spots array, a prism for 2-dimensional (2-D) spectral dispersion and a specially designed picosecond streak camera for providing simultaneous temporal and spectral resolutions, which enables the acquisition of fluorescence lifetime information of any spectrum within the whole spectra in only one measurement. The near-infrared light from a mode-locked titanium: sapphire femtosecond laser is split into an array of beams that are transformed into an array of high-aperture foci at the sample. A time- and spectrum-resolved image of 3×3 foci on the sample can be obtained with a snapshot. By translating the sample stage laterally and axially and implementing a dedicated image reconstruction algorithm in the control system, 5-D data sets that combine temporal and spectral resolutions and 3-dimensional (3-D) spatial coordinates can be obtained. The spectrum-resolved and depth-resolved fluorescence lifetime microscopic images are demonstrated. Preliminary results indicate that the system has high temporal, spectral and spatial resolutions and holds potential for providing high image acquisition rate.

Citation Download Citation

Lixin Liu, Junle Qu D.D.S., Lei Wang, Ziyang Lin, Zhe Fu, Baoping Guo, Wenqing Liu, and Hanben Niu "Five-dimensional multifocal multiphoton microscopy", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341Q (1 May 2007); https://doi.org/10.1117/12.741580

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