Total internal reflection energy transfer (TIRET) microscopy for analysis of focal adhesions in living cells

Brigitte Angres; Heiko Steuer; Michael Wagner; Petra Weber; Herbert Schneckenburger

doi:10.1117/12.662073

14 April 2006 Total internal reflection energy transfer (TIRET) microscopy for analysis of focal adhesions in living cells

Brigitte Angres, Heiko Steuer, Michael Wagner, Petra Weber, Herbert Schneckenburger

Author Affiliations +

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61910A (2006) https://doi.org/10.1117/12.662073
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

Total internal reflection fluorescence microscopy (TIRFM) is used to measure non-radiative energy transfer between membrane associated proteins in living cells. Measurements are concentrated on focal contacts and their associated proteins focal adhesion kinase (FAK) and Paxillin (Pax) which play major roles with respect to cell migration, growth, and survival. These proteins are visualized after fusion with variants of green fluorescent protein (ECFP and EYFP), and an intermolecular energy transfer ECFP -> EYFP is deduced from fluorescence spectra as well as from fluorescence decay kinetics of single cells.

Citation Download Citation

Brigitte Angres, Heiko Steuer, Michael Wagner, Petra Weber, and Herbert Schneckenburger "Total internal reflection energy transfer (TIRET) microscopy for analysis of focal adhesions in living cells", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910A (14 April 2006); https://doi.org/10.1117/12.662073

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