Mr. Michael Wagner Profile

Michael Wagner

at Hochschule Aalen

SPIE Involvement:

Author

Publications (31)

Proceedings Article | 30 July 2024 Paper

Round-robin test of subsurface damage characterization in ZERODUR using non-destructive optical coherence tomography

S. Frank, L. Schwörer, V. Alvan, P. Hohmann, I. Burger, S. Jedamzik, D. Thelemann, M. Wagner, J. Bliedtner, R. Börret

Proceedings Volume 13221, 1322102 (2024) https://doi.org/10.1117/12.3031491

KEYWORDS: Lens grinding, Optical coherence tomography, Zerodur, 3D metrology, Nondestructive evaluation, Manufacturing, Glass ceramics, Optics manufacturing

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The glass ceramic ZERODUR® with its extremely low thermal expansion finds widespread application in precision optics and astronomical instruments. It has been used for several very large telescope mirrors, as its unique properties make it indispensable in environments demanding stringent dimensional stability. As for all brittle materials, mechanical grinding processes involved in manufacturing introduce subsurface damage (SSD), which compromises the optical and mechanical performance of workpieces. Beyond its implications for quality, SSD is often a significant cost driver, necessitating expensive finishing processes. Characterizing SSD and determining its depth at various manufacturing stages from pre-grinding to final polishing are critical for ensuring the quality and reliability of optical components. Conventional measurement techniques predominantly involve destructive methods such as taper polishing or cleaving the surface, posing limitations in assessing SSD without compromising the integrity of the workpiece. Moreover, these methods are often time-consuming and labor-intensive. In a collaborative study, we conducted a round-robin test to establish and validate the efficacy of nondestructive optical coherence tomography (OCT) as a new technique for characterizing SSD depths in ground ZERODUR®. The aim is to provide a first comprehensive assessment of the capability of OCT in nondestructively quantifying SSD in glass ceramics. The methodology involves comparing measurements obtained from two distinct high-resolution OCT systems applied to identical ZERODUR® samples machined with selected process parameters. Furthermore, the study investigates the influence of different grain sizes on the SSD characteristics by comparing measurements across samples processed with varying grain sizes. By conducting the round-robin test, we seek to demonstrate the applicability of OCT as a non-destructive alternative for SSD characterization in ground ZERODUR®. Successful validation of OCT extended to a variety of relevant optic materials such as ZERODUR® would enhance overall quality control processes, offering a rapid and non-invasive means of assessing SSD depths and ensuring the integrity of critical optical components.

Proceedings Article | 9 September 2019 Paper

Combining TIR and FRET: from fluorescence microscopy to a multi-well reader system

Herbert Schneckenburger, Petra Weber, Michael Wagner, Sandra Enderle, Julian Weghuber, Peter Lanzerstorfer

Proceedings Volume 11076, 110761C (2019) https://doi.org/10.1117/12.2526416

KEYWORDS: Luminescence, Fluorescence resonance energy transfer, Microscopy, Fluorescence lifetime imaging, Molecules, Molecular interactions, Green fluorescent protein, Energy transfer, Picosecond phenomena, Plasma

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Proceedings Article | 17 May 2018 Presentation + Paper

Structured illumination for live cell microscopy

Verena Richter, Mathis Piper, Michael Wagner, Herbert Schneckenburger

Proceedings Volume 10685, 106852J (2018) https://doi.org/10.1117/12.2306219

KEYWORDS: Microscopy, Objectives, Luminescence, Microscopes, Breast cancer, Spatial frequencies, Spatial light modulators, Filtering (signal processing), Image resolution, Super resolution

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Proceedings Article | 28 July 2017 Paper

Axial tomography in 3D live cell microscopy

Verena Richter, Sarah Bruns, Thomas Bruns, Mathis Piper, Petra Weber, Michael Wagner, Christoph Cremer, Herbert Schneckenburger

Proceedings Volume 10414, 104140P (2017) https://doi.org/10.1117/12.2286602

KEYWORDS: Tomography, Microscopy

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SPIE Journal Paper | 25 January 2017 Open Access

Axial tomography in live cell laser microscopy

Verena Richter, Sarah Bruns, Thomas Bruns, Petra Weber, Michael Wagner, Christoph Cremer, Herbert Schneckenburger

JBO, Vol. 22, Issue 09, 091505, (January 2017) https://doi.org/10.1117/12.10.1117/1.JBO.22.9.091505

KEYWORDS: Microscopy, Tomography, Luminescence, Green fluorescent protein, Capillaries, Objectives, 3D image processing, Quantum dots, Glasses, Solids

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Proceedings Article | 15 July 2015 Paper

3D imaging of apoptosis by FRET, light sheet fluorescence and scattering microsopy

Herbert Schneckenburger, Petra Weber, Sarah Schickinger, Verena Richter, Thomas Bruns, Michael Wagner

Proceedings Volume 9537, 953716 (2015) https://doi.org/10.1117/12.2183607

KEYWORDS: Luminescence, Cell death, Light scattering, Microscopy, Scattering, Fluorescence resonance energy transfer, Energy transfer, Fluorescent proteins, Spatial resolution, Sensors

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SPIE Journal Paper | 16 December 2013 Open Access

Nanosecond ratio imaging of redox states in tumor cell spheroids using light sheet-based fluorescence microscopy

Sarah Schickinger, Thomas Bruns, Herbert Schneckenburger, Rainer Wittig, Petra Weber, Michael Wagner

JBO, Vol. 18, Issue 12, 126007, (December 2013) https://doi.org/10.1117/12.10.1117/1.JBO.18.12.126007

KEYWORDS: Luminescence, Microscopy, Tumors, Cameras, Sensors, 3D image processing, Confocal microscopy, Cell death, Microfluidics, Semiconductor lasers

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Proceedings Article | 18 June 2013 Paper

Visualizing a cytostatic drug and probing apoptosis of cancer cells

Verena Richter, Petra Weber, Michael Wagner, Sarah Schickinger, Thomas Bruns, Herbert Schneckenburger

Proceedings Volume 8798, 879804 (2013) https://doi.org/10.1117/12.2032461

KEYWORDS: Luminescence, Microscopy, Light scattering, Scattering, Cell death, Microscopes, Breast cancer, Mie scattering, Visualization, Spatial resolution

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SPIE Journal Paper | 11 June 2012 Open Access

Tumor cell differentiation by label-free fluorescence microscopy

Petra Weber, Michael Wagner, Herbert Schneckenburger, Petra Kioschis, Waltraud Kessler

JBO, Vol. 17, Issue 10, 101508, (June 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.10.101508

KEYWORDS: Luminescence, Tumors, Microscopy, Principal component analysis, Phase contrast, Imaging spectroscopy, Molybdenum, Mode conditioning cables, Fluorescence lifetime imaging, Picosecond phenomena

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Proceedings Article | 11 February 2011 Paper

Tumor cell differentiation by marker free fluorescence microscopy

Herbert Schneckenburger, Petra Weber, Michael Wagner, Marco Brantsch, Philipp Biller, Petra Kioschis, Waltraud Kessler

Proceedings Volume 7902, 79020D (2011) https://doi.org/10.1117/12.874655

KEYWORDS: Luminescence, Raman spectroscopy, Tumors, Data analysis, Raman scattering, Picosecond phenomena, Microscopy, Proteins, Phase contrast, Tissues

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Proceedings Article | 25 November 2010 Paper

Microfluidic system for single cell sorting with optical tweezers

Thomas Bruns, Laszlo Becsi, Marc Talkenberg, Michael Wagner, Petra Weber, Ulrich Mescheder, Herbert Schneckenburger

Proceedings Volume 7376, 73760M (2010) https://doi.org/10.1117/12.871450

KEYWORDS: Optical tweezers, Microfluidics, Glasses, Silicon, Plating, Polymethylmethacrylate, Polymers, Laser applications, Ovary, Nd:YAG lasers

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SPIE Journal Paper | 1 July 2010 Open Access

Fluorescence imaging of membrane dynamics in living cells

Petra Weber, Michael Wagner, Herbert Schneckenburger

JBO, Vol. 15, Issue 04, 046017, (July 2010) https://doi.org/10.1117/12.10.1117/1.3470446

KEYWORDS: Luminescence, Plasma, Fluorescence anisotropy, Anisotropy, Polarization, Microscopy, Cameras, Temperature metrology, Imaging spectroscopy, Microscopes

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Proceedings Article | 8 July 2009 Paper

A membrane-associated FRET sensor for detection of apoptosis

Herbert Schneckenburger, Michael Wagner, Petra Weber, Thomas Bruns, Heiko Steuer, Brigitte Angres

Proceedings Volume 7368, 73680Q (2009) https://doi.org/10.1117/12.831570

KEYWORDS: Luminescence, Cell death, Fluorescence resonance energy transfer, Glasses, Fluorescent proteins, Sensors, Microscopy, Energy transfer, Computer programming, Microscopes

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Proceedings Article | 1 July 2009 Paper

Dose limited fluorescence microscopy of living cells

Herbert Schneckenburger, Michael Wagner, Petra Weber, Sarah Schickinger, Thomas Bruns, Wolfgang S. Strauss

Proceedings Volume 7367, 73670E (2009) https://doi.org/10.1117/12.831547

KEYWORDS: Microscopy, Luminescence, Plating, Laser scanners, Optical microscopy, Multiphoton microscopy, Stimulated emission depletion microscopy, Cameras, Control systems, Plasma

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Proceedings Article | 1 July 2009 Paper

Tomographic screening of 3-dimensional cell cultures

Thomas Bruns, Verena Richter, Michael Wagner, Wolfgang S. Strauss, Herbert Schneckenburger

Proceedings Volume 7367, 73671S (2009) https://doi.org/10.1117/12.831546

KEYWORDS: Image resolution, Microscopes, Objectives, Tomography, Luminescence, Microscopy, 3D image processing, Fluorescence spectroscopy, Lenses, 3D image reconstruction

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SPIE Journal Paper | 1 March 2009 Open Access

Förster resonance energy transfer-based total internal reflection fluorescence reader for apoptosis

Thomas Bruns, Brigitte Angres, Heiko Steuer, Petra Weber, Michael Wagner, Herbert Schneckenburger

JBO, Vol. 14, Issue 02, 021003, (March 2009) https://doi.org/10.1117/12.10.1117/1.3055622

KEYWORDS: Luminescence, Cell death, Glasses, Fluorescence resonance energy transfer, Energy transfer, Fluorescent proteins, Electromagnetism, Microelectromechanical systems, Molecules, Microscopes

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Proceedings Article | 18 February 2009 Paper

Dose limited fluorescence microscopy of 5-aminolevulinic acid induced protoporphyrin IX in living cells

H. Schneckenburger, P. Weber, M. Wagner, S. Schickinger, T. Bruns, W. S. Strauss

Proceedings Volume 7164, 71640F (2009) https://doi.org/10.1117/12.808813

KEYWORDS: Microscopy, Luminescence, Photodynamic therapy, Laser scanners, Electromagnetism, Plasma, Argon ion lasers, Semiconductor lasers, Microscopes, Objectives

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Proceedings Article | 12 February 2009 Paper

Fluorescence and polarization imaging of membrane dynamics in living cells

M. Wagner, P. Weber, T. Bruns, W. S. Strauss, H. Schneckenburger

Proceedings Volume 7176, 717607 (2009) https://doi.org/10.1117/12.808788

KEYWORDS: Luminescence, Plasma, Polarization, Fluorescence anisotropy, Anisotropy, Microscopy, Microscopes, Time resolved spectroscopy, Fluid dynamics, Microfluidics

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Proceedings Article | 13 July 2007 Paper

Fluorescence imaging of cholesterol and temperature dependent cell membrane dynamics

Petra Weber, Michael Wagner, Wolfgang Strauss, Herbert Schneckenburger

Proceedings Volume 6633, 663305 (2007) https://doi.org/10.1117/12.728136

KEYWORDS: Luminescence, Polarization, Plasma, Microscopy, Molecules, Liquids, Crystals, Liquid crystals, Microscopes, Temperature metrology

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Proceedings Article | 13 July 2007 Paper

Axially resolved polarisation microscopy of membrane dynamics in living cells

Michael Wagner, Petra Weber, Herbert Schneckenburger

Proceedings Volume 6633, 663309 (2007) https://doi.org/10.1117/12.727780

KEYWORDS: Luminescence, Plasma, Anisotropy, Fluorescence anisotropy, Microscopy, Temperature metrology, Time resolved spectroscopy, Polarization, Diffusion, 3D modeling

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Proceedings Article | 19 February 2007 Paper

TIRET microscopy: monitoring protein (amyloid precursor protein and beta-secretase) interaction on the surface of living cells

Christine von Arnim, Michael Wagner, Petra Weber, Herbert Schneckenburger

Proceedings Volume 6441, 64410D (2007) https://doi.org/10.1117/12.699856

KEYWORDS: Luminescence, Computer programming, Proteins, Microscopy, Energy transfer, Magnesium, Green fluorescent protein, Fluorescence resonance energy transfer, Plasma, Picosecond phenomena

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Proceedings Article | 19 February 2007 Paper

Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)

Herbert Schneckenburger, Harald Baumann, Michael Wagner, Wolfgang Strauss

Proceedings Volume 6441, 64411E (2007) https://doi.org/10.1117/12.699826

KEYWORDS: Luminescence, Modulation, Microscopy, Microscopes, Reflection, Prisms, Objectives, Mirrors, Interfaces, Glasses

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SPIE Journal Paper | 1 May 2006 Open Access

Total internal reflectance fluorescence reader for selective investigations of cell membranes

Thomas Bruns, Wolfgang Strauss, Reinhard Sailer, Michael Wagner, Herbert Schneckenburger

JBO, Vol. 11, Issue 03, 034011, (May 2006) https://doi.org/10.1117/12.10.1117/1.2208617

KEYWORDS: Luminescence, Glasses, Plasma, Reflectivity, Proteins, Breast cancer, CCD cameras, Adhesives, Refractive index, Fluorescence spectroscopy

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Proceedings Article | 14 April 2006 Paper

Microspectrofluorometry and polarization microscopy of membrane dynamics in living cells

Michael Wagner, Petra Weber, Herbert Schneckenburger

Proceedings Volume 6191, 619109 (2006) https://doi.org/10.1117/12.662023

KEYWORDS: Luminescence, Polarization, Plasma, Diffusion, Microscopy, Microscopes, Anisotropy, Fluid dynamics, Molecules, Temperature metrology

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Proceedings Article | 14 April 2006 Paper

Total internal reflection energy transfer (TIRET) microscopy for analysis of focal adhesions in living cells

Brigitte Angres, Heiko Steuer, Michael Wagner, Petra Weber, Herbert Schneckenburger

Proceedings Volume 6191, 61910A (2006) https://doi.org/10.1117/12.662073

KEYWORDS: Luminescence, Proteins, Energy transfer, Microscopy, Image fusion, Computer programming, Green fluorescent protein, Fluorescence resonance energy transfer, Visualization, Fluorescent proteins

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Proceedings Article | 8 October 2005 Paper

TIR fluorescence reader for selective detection of cell membranes

Thomas Bruns, Wolfgang Strauss, Reinhard Sailer, Michael Wagner, Herbert Schneckenburger

Proceedings Volume 5859, 585908 (2005) https://doi.org/10.1117/12.632828

KEYWORDS: Luminescence, Glasses, Proteins, CCD cameras, Plasma, Breast cancer, Reflection, Adhesives, Optical filters, Refractive index

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Proceedings Article | 8 April 2005 Paper

Photodynamic efficacy and spectroscopic properties of 5-ALA-induced protoporphyrin IX in human glioblastoma cells

Reinhard Sailer, Wolfgang Strauss, Helmut Emmert, Michael Wagner, Rudolf Steiner, Herbert Schneckenburger

Proceedings Volume 5689, (2005) https://doi.org/10.1117/12.590120

KEYWORDS: Luminescence, Breast cancer, Ovarian cancer, Molecules, Brain, Fluorescence lifetime imaging, Photodynamic therapy, Spectroscopy, Fluorescence spectroscopy, Picosecond phenomena

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Proceedings Article | 29 March 2005 Paper

Cholesterol dependence of cell membrane dynamics

Petra Weber, Michael Wagner, Reinhard Sailer, Wolfgang Strauss, Herbert Schneckenburger

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.590236

KEYWORDS: Luminescence, Polarization, Plasma, Microscopy, Temperature metrology, Diffusion, Fluorescence anisotropy, Picosecond phenomena, Molecules, Microscopes

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Proceedings Article | 29 March 2005 Paper

TIR fluorescence screening of cell membranes

Thomas Bruns, Wolfgang Strauss, Reinhard Sailer, Michael Wagner, Herbert Schneckenburger

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.590199

KEYWORDS: Luminescence, Glasses, Reflection, Proteins, CCD cameras, Breast cancer, Optical filters, Refractive index, Adhesives, Fluorescence spectroscopy

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Proceedings Article | 9 October 2003 Paper

Fluorescence lifetime imaging (FLIM) of membrane markers in living cells

Herbert Schneckenburger, Michael Wagner, Martina Kretzschmar, Wolfgang Strauss, Reinhard Sailer

Proceedings Volume 5139, (2003) https://doi.org/10.1117/12.502245

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Plasma, Breast cancer, Picosecond phenomena, Molecules, Microscopy, Rhodamine, Microscopes, Liquids

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Proceedings Article | 19 June 2003 Paper

Time-resolved spectroscopic and microscopic measurements of membrane dynamics in living cells

Herbert Schneckenburger, Martina Kretzschmar, Michael Wagner, Wolfgang Strauss, Reinhard Sailer

Proceedings Volume 4962, (2003) https://doi.org/10.1117/12.478118

KEYWORDS: Luminescence, Plasma, Polarization, Molecules, Spectroscopy, Time resolved spectroscopy, Microscopy, Picosecond phenomena, Microscopes, Temperature metrology

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Showing 5 of 31 publications

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