Development of microfluidic structures for high throughput flow cytometric characterization of blood cells

A. Kummrow; H. Yildirim; J. Theisen; K. Brattke; C. Sprenger; M. Schmidt; J. Neukammer

doi:10.1117/12.728440

13 July 2007 Development of microfluidic structures for high throughput flow cytometric characterization of blood cells

A. Kummrow, H. Yildirim, J. Theisen, K. Brattke, C. Sprenger, M. Schmidt, J. Neukammer

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66332C (2007) https://doi.org/10.1117/12.728440
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

We exploited ultraprecision milling to fabricate three dimensional microfluidic structures incorporating cascaded hydrodynamic focusing. Stable operation when varying the sample flow over three orders of magnitude was demonstrated. Impedance detection or forward light scatter served to detect polystyrene microspheres and to differentiate red blood cells and blood platelets.

Citation Download Citation

A. Kummrow, H. Yildirim, J. Theisen, K. Brattke, C. Sprenger, M. Schmidt, and J. Neukammer "Development of microfluidic structures for high throughput flow cytometric characterization of blood cells", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66332C (13 July 2007); https://doi.org/10.1117/12.728440

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