Targeting the human serotonin transporter (hSERT) with quantum dots

I. D. Tomlinson; Jerry Chang; Hideki Iwamoto; Louis J. De Felice; Randy D. Blakely; Sandra J. Rosenthal

doi:10.1117/12.782801

26 February 2008 Targeting the human serotonin transporter (hSERT) with quantum dots

I. D. Tomlinson, Jerry Chang, Hideki Iwamoto, Louis J. De Felice, Randy D. Blakely, Sandra J. Rosenthal

Proceedings Volume 6866, Colloidal Quantum Dots for Biomedical Applications III; 68660X (2008) https://doi.org/10.1117/12.782801
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this paper we report our work on the development of a human serotonin transporter (hSERT) antagonist that can be conjugated to quantum dots. This approach has been used to target and visualize the human serotonin transporter protein (hSERT). We demonstrate that labeling is blocked by the addition of high affinity hSERT antagonists such as paroxetine. This approach may be useful for the development of fluorescent assays to study the location and temporal dynamics of biogenic amine transporters and also holds promise for the development of plate-based high throughput assays used to identify novel transporter antagonists.

Citation Download Citation

I. D. Tomlinson, Jerry Chang, Hideki Iwamoto, Louis J. De Felice, Randy D. Blakely, and Sandra J. Rosenthal "Targeting the human serotonin transporter (hSERT) with quantum dots", Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660X (26 February 2008); https://doi.org/10.1117/12.782801

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