Paper
25 February 2010 Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy
Patrick Nuernberger, Kevin F. Lee, Adeline Bonvalet, Antigoni Alexandrou, Marten H. Vos, Manuel Joffre
Author Affiliations +
Abstract
We report on ultrafast visible pump/mid-infrared probe spectroscopy of the carboxy form of heme proteins by employing the recently developed chirped-pulse upconversion technique, which allows both high resolution and sensitivity over an extremely broad spectral range. Commonly, the bleach signal due to ligand dissociation and the incipient docking-site absorption signal, being about 200 cm-1 apart and differing by more than an order of magnitude in absorptivity, are studied in separate experiments. We here monitor them simultaneously, allowing a direct observation and a concurrent analysis of the initial processes after photoinduced ligand dissociation, for instance, the formation of hot vibrational bands.
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Patrick Nuernberger, Kevin F. Lee, Adeline Bonvalet, Antigoni Alexandrou, Marten H. Vos, and Manuel Joffre "Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy", Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600F (25 February 2010); https://doi.org/10.1117/12.843672
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KEYWORDS
Absorption

Mid-IR

Proteins

Spectroscopy

Visible radiation

Ultrafast laser spectroscopy

Infrared spectroscopy

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