Picosecond polarization spectroscopy of fluorescein attached to different molecular volume polymer influenced by rotational motion

Yang Pu; Wubao Wang; R. B. Dorshow; R. R. Alfano

doi:10.1117/12.904692

20 February 2012 Picosecond polarization spectroscopy of fluorescein attached to different molecular volume polymer influenced by rotational motion

Yang Pu, Wubao Wang, R. B. Dorshow, R. R. Alfano

Author Affiliations +

Proceedings Volume 8258, Organic Photonic Materials and Devices XIV; 825818 (2012) https://doi.org/10.1117/12.904692
Event: SPIE OPTO, 2012, San Francisco, California, United States

Abstract

Time-resolved fluorescence polarization spectroscopy of various molecular volume fluorescein dye-labeled polymers influenced by the molecular rotation motion was investigated by picosecond dynamics measurements. The time-resolved picosecond polarization spectroscopy was modeled using a system of first-order linear differential equations containing two main parameters: the decay rate of emission and the rate of one orthogonal emission component transferring to another. Both experimental observation and theoretical calculation indicate that the fluorescent molecular rotation is the cause of the depolarization. The dipole's rotational time was extracted and shows increasing with the molecular volume obeying the Einstein-Stokes relation.

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Yang Pu, Wubao Wang, R. B. Dorshow, and R. R. Alfano "Picosecond polarization spectroscopy of fluorescein attached to different molecular volume polymer influenced by rotational motion", Proc. SPIE 8258, Organic Photonic Materials and Devices XIV, 825818 (20 February 2012); https://doi.org/10.1117/12.904692

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