Nanoscopy with focused light

Stefan W. Hell

doi:10.1117/12.2032261

10 June 2013 Nanoscopy with focused light

Stefan W. Hell

Proceedings Volume 8882, ROMOPTO 2012: Tenth Conference on Optics: Micro- to Nanophotonics III; 888203 (2013) https://doi.org/10.1117/12.2032261
Event: ROMOPTO International Conference on Micro- to Nano-Photonics III, 2012, Bucharest, Romania

Abstract

For many decades, it has been accepted that the resolution of a lens-based optical microscope is limited to about d =λ (2 NA) < 200 nm. The discovery in the 1990’s that elementary transitions between the states of a fluorophore can be used to eliminate the limiting role of diffraction has led to lens-based light microscopy concepts with resolution down to the nanometer scale^1,2. Currently, all far-field fluorescence nanoscopy (superresolution) concepts that have found wider application share a common enabling element: they modulate the fluorescence capability of adjacent features such that they fluoresce sequentially^3,4.

Citation Download Citation

Stefan W. Hell "Nanoscopy with focused light", Proc. SPIE 8882, ROMOPTO 2012: Tenth Conference on Optics: Micro- to Nanophotonics III, 888203 (10 June 2013); https://doi.org/10.1117/12.2032261

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