Detection of back-reflected SHG from corneal histological sections

Ana Batista; António Miguel Morgado; Karsten König

doi:10.1117/12.2038251

28 February 2014 Detection of back-reflected SHG from corneal histological sections

Ana Batista, António Miguel Morgado, Karsten König

Proceedings Volume 8948, Multiphoton Microscopy in the Biomedical Sciences XIV; 89482B (2014) https://doi.org/10.1117/12.2038251
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The feasibility of using reflective surfaces to increase the detection of SHG signals from corneal histological sections in the backward direction was attempted. Three reflective surfaces were tested: aluminum foil, a silver mirror, and a dielectric mirror with specific high reflectivity around 400 nm. To compare the SHG signal detected with and without reflective surfaces, the translation caused by the bending of the sample due to the extra weight on top of the microscope slide was determined. All reflective surfaces resulted in an increase of the detection of SHG signals. Increases of 4%, 11%, and 16% were observed for aluminum foil, dielectric mirror and silver mirror, respectively. A method for increased detection of backward SHG signals when forward detection is not possible was herein demonstrated.

Citation Download Citation

Ana Batista, António Miguel Morgado, and Karsten König "Detection of back-reflected SHG from corneal histological sections", Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482B (28 February 2014); https://doi.org/10.1117/12.2038251

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